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Part:BBa_K5230002

Designed by: Minyi Yu   Group: iGEM24_UToronto   (2024-09-29)


Origin of replication for pColK_K235 PCR product

Origin of replication for the E. coli plasmid pColK_K235. PCR results indicated that BBa_K5230002 had a size 1.4 kb.

The ori sequence underwent PCR with the pColK_K235_RNAII_A_F and pColK_K235_RR_C_R primers.

It was annealed to the plasmid backbone eforRed/eforCP(conferring ampicillin resistance) via Golden Gate Assembly, transformed into E. coli, and plated. Growth was observed when annealed with eforRed/eforCP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4
    Illegal BsaI.rc site found at 1362


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