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Part:BBa_K5230002
Designed by: Minyi Yu Group: iGEM24_UToronto (2024-09-29)
Origin of replication for pColK_K235 PCR product
Origin of replication for the E. coli plasmid pColK_K235. PCR results indicated that BBa_K5230002 had a size 1.4 kb.
The ori sequence underwent PCR with the pColK_K235_RNAII_A_F and pColK_K235_RR_C_R primers.
It was annealed to the plasmid backbone eforRed/eforCP(conferring ampicillin resistance) via Golden Gate Assembly, transformed into E. coli, and plated. Growth was observed when annealed with eforRed/eforCP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4
Illegal BsaI.rc site found at 1362
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